|
KMID : 0900919970210030239
|
|
Korean journal of Animal Reproduction
1997 Volume.21 No. 3 p.239 ~ p.246
|
|
|
Differences in Polyspermy and Penetration Rate Following In Vitro Fertilization of In Vitro Matured Porcine Oocytes by Morphologically Normal Sperm Ratio of Epididymal Sperm and Frozen-Thawed Ejaculated Sperm
|
|
Lee Hyun-Sook
Kim I. C.
Choi Dong-Yoon
Kim Eun-Young
|
|
|
Abstract
|
|
|
|
The objective of this study was to evaluate the variation of fertilizing ability following the morphologically normal sperm ratio in porcine IVF using epididymal sperm The results obtained in this experiment were summarized as follows: 1. When the penetration rate (PR), polysper my rate (PSR), pronuclei formation (2PNF) and mean number of sperm (MNS) per oocyte were evaluated according to the percentage of morphologically normal epididyrnal sperm at insemination(leqlO%, 10~30% and geq50%). the PR and PSR of leq50% group (82.4, 87.4%) were significantly higher than those of other two groups (leqlO%; 29.7%, 22.6% and 10~30%; 20.3, 37.0%) (p<0.01). Also, the 2PNF per examined oocytes was significantly high in geq 50% group (p<0.01). 2. When the geq50% group in epididymal sperm was adjusted to 100% (5x1^5 cells/ml) , the PSR and 2PNF were not different between epididymal sperm (86.7, 35.1%) and frozen-thawed ejaculated sperm (86.0. 39.4%) although the PR in epididymal sperm (79.7%) was significantly lower than that in frozen-thawed ejaculated sperm (95.5%)(p<0.01). 3. Also. when the PR, PSR, 2PNF and MNS of epididymal sperm were evaluated according to the oocyte: sperm ratio (1:6000, 1:6650. 1:7700 and 1: 10000) at insemination. the PR, PSR and MNS were increased as the oocyte:sperm ratio increases. However, this result indicated that the 2PNF was high in the oocyte:sperm ratio (1:6000 and 1:6650). Therefore. these results suggested that when the percentage of morphologically normal epididymal sperm was more than 50. the fertilizing a ability was very similar to that of frozen-thawed ejaculated sperm and that the detailed evalu￢a ation of morphological normality in porcine IVF using epididymal sperm should be prerequisite to obtain the more effective fertilizing ability.
|
|
|
KEYWORD
|
|
|
Porcine, Epididymal/Frozen-thawed ejaculated sperm, Fertilizing ability
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|